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Immunofluorescent staining of human cell line A-431 shows localization to vesicles.
Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus & vesicles.
Antibody dilution
1:35
1:50
Literature conformity
The subcellular location is not consistent with literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA027405
Antibody HPA027459
Antibody HPA027463
Standard validation
Approved
Approved
Approved
Figure description
Immunohistochemical staining of human lateral ventricle shows strong cytoplasmic positivity in neurons.
Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 57 cell types
RNA: detected in 37 tissues Protein: detected in 59 cell types
RNA: detected in 37 tissues Protein: detected in 29 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:15
1:125
1:40
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA027405
Antibody HPA027459
Antibody HPA027463
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
143.2, 134.5
143.2, 134.5
143.2
Antibody dilution
1:250
1:250
1:250
PROTEIN ARRAY
Antibody HPA027405
Antibody HPA027459
Antibody HPA027463
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
