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Immunohistochemical staining of adrenal gland shows strong cytoplasmic positivity in medullary cells.
Immunohistochemical staining of adrenal gland shows strong cytoplasmic positivity in medullary cells.
Immunohistochemical staining of adrenal gland shows strong cytoplasmic positivity in medullary cells.
Expression
RNA: detected in 2 tissues Protein: detected in 2 cell types
RNA: detected in 2 tissues Protein: detected in 2 cell types
RNA: detected in 2 tissues Protein: detected in 19 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:1000
1:50
1:10000
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Consistent with RNA expression data.
Mainly consistent with RNA expression data.
Standard validation
Supported
Figure description
Staining of dopaminergic and noradrenergic neurons, their processes and synapses. Strongest immunoreactivity in the caudate putamen, substantial nigra compact and locus coeruleus.
WESTERN BLOT
Antibody HPA061003
Antibody CAB002522
Antibody CAB072340
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Current setup is not applicable due to low RNA count.
Uncertain
Analysis performed using a standard panel of samples. Current setup is not applicable due to low RNA count.
Uncertain
Analysis performed using a standard panel of samples. Current setup is not applicable due to low RNA count.
Figure description
Target mass (kDa)
58.6, 58.2, 55.6, 44.9
58.6, 58.2, 55.6, 44.9, 13
58.6, 58.2, 55.6, 44.9, 13
Antibody dilution
1:480
1:500
1:500
PROTEIN ARRAY
Antibody HPA061003
Antibody CAB002522
Antibody CAB072340
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
