Fields »
ABOUT
HELP
BLOG
GLUD2

ANTIBODY INFORMATION
 
 

Antibody HPA042492

  

Antibody HPA044839

  

Antibody HPA061369

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA042492   HPA044839   HPA061369  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

 GLUD1 - ENSG00000148672 [100%]
   GLUD1 - ENSG00000148672 [100%]
   GLUD1 - ENSG00000148672 [100%]
  

Released in version

 10   10   16  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA042492

  

Antibody HPA044839

  

Antibody HPA061369

  
Immunocytochemistry

  Supported   Supported  
Immunohistochemistry

Uncertain   Uncertain    
Western blot

Supported   Supported   Supported  
Protein array

Supported   Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA042492

  

Antibody HPA044839

  

Antibody HPA061369

  

Standard validation

   Supported   Supported  

     

Figure description

   Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.   Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.  

Antibody dilution

   1:84   1:63  

Literature conformity

   The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA042492

  

Antibody HPA044839

  

Antibody HPA061369

  

Standard validation

 Uncertain   Uncertain    

     

Figure description

 Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in cells in seminiferus ducts and Leydig cells.   Immunohistochemical staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.    

Expression

 RNA: detected in 9 tissues
Protein: detected in 58 cell types		   RNA: detected in 9 tissues
Protein: detected in 75 cell types		    

Retrieval

 HIER pH6   HIER pH6    

Antibody dilution

 1:1000   1:2000    

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.    

RNA consistency

 Not consistent with RNA expression data.   Not consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA042492

  

Antibody HPA044839

  

Antibody HPA061369

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 61.4   61.4   61.4  

Antibody dilution

 1:250   1:250   1:210  

PROTEIN ARRAY
 
 

Antibody HPA042492

  

Antibody HPA044839

  

Antibody HPA061369

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:6000   1:3000   1:2500  

ANTIGEN INFORMATION
 
 

Antibody HPA042492

  

Antibody HPA044839

  

Antibody HPA061369

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 83   70   78  

Antigen sequence

 
KPCNHVLSLSFPIRRDDGSWEVIEGYRAQHSQHRTPCKGGIRYSTDVSVD
EVKALASLMTYKCAVVDVPFGGAKAGVKINPKN
  
TFVVQGFGNVGLHSMRYLHRFGAKCIAVGESDGSIWNPDGIDPKELEDFK
LQHGSILGFPKAKPYEGSIL
  
DMSTGEREMSWIADTYASTIGHYDINAHACVTGKPISQGGIHGRISATGR
GVFHGIENFINEASYMSILGMTPGFGDK
 

Matching transcripts

 GLUD2-001 - ENSP00000327589 [100%]
  GLUD2-001 - ENSP00000327589 [100%]
  GLUD2-001 - ENSP00000327589 [97%]
 

Other gene match

 GLUD1 - ENSG00000148672 [100%]
   GLUD1 - ENSG00000148672 [100%]
   GLUD1 - ENSG00000148672 [100%]
  
ANTIGEN VIEW
GLUD2-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.