Cell atlas - ALYREF - The Human Protein Atlas

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ALYREF

TISSUE

CELL

CANCER

CELL ATLAS RNA EXPRESSION HUMAN CELLS siRNA

GENE/PROTEIN

Antibody validation

Dictionary
	Nuclear speckles

Human cell
	Nucleoplasm

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GENERAL INFORMATION
Gene name	ALYREF
Gene description	Aly/REF export factor
Protein class	Predicted intracellular proteins
Predicted localization	Intracellular
Number of transcripts	1
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HUMAN PROTEIN ATLAS INFORMATION
Summary	Mainly localized to the nuclear speckles.
RNA cell category	Expressed in all
Protein evidence	Evidence at protein level
Main location	Localized to the Nuclear speckles (validated)
siRNA	The location(s) are Validated based on siRNA mediated gene silencing.

DATA RELIABILITY
Reliability score	Validated based on 3 antibodies. CAB016281 , HPA019799 , HPA061282
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ASSAYS

RNA expression

Human cells

siRNA

RNA EXPRESSION OVERVIEW
RNA cell line category: Expressed in all Organ Origin Category Expression Alphabetical Cell lines sorted after organ of phenotypic resemblance. Cell lines sorted after biological source for establishment. Cell lines sorted after the cell line category according to Cellosaurus. Cell lines sorted on descending RNA expression. Cell lines sorted alphabetically.

HUMAN CELLS

Summary

Mainly localized to the nuclear speckles.

Main location

Nuclear speckles (validated)


	Toggle channels	Low High G1 S G2 M N/A

Antibody	Cell line	Location
CAB016281	A-431	Nuclear speckles
CAB016281	U-2 OS	Nucleus
CAB016281	U-2 OS	Nucleoli
CAB016281	U-251 MG	Nucleus
HPA019799	A-431	Nuclear speckles
HPA019799	U-2 OS	Nuclear speckles
HPA019799	U-251 MG	Nuclear speckles
HPA061282	HEK 293	Nucleus
HPA061282	HEK 293	Vesicles
HPA061282	U-2 OS	Nuclear speckles
HPA061282	U-251 MG	Nucleus

siRNA VALIDATION

Relative fluorescence intensity (RFI)

Antibody

HPA019799

siRNA validation

Validated: Significant downregulation > 25% by one siRNA.

Analysis performed in

Nuclear region of segmented cells in 10x-images

siRNA 2

RFI

35%

p-value

< 0.01


	Toggle channels	Low High G1 S G2 M N/A

Thumbnail 10x	Thumbnail 40x	Antibody	Sample	Cell line
		HPA019799	siRNA 2	U-2 OS
		HPA019799	scrambled	U-2 OS

GENE INFORMATION
Gene name	ALYREF (HGNC Symbol)
Synonyms	ALY, ALY/REF, BEF, REF, THOC4
Description	Aly/REF export factor (HGNC Symbol)
Entrez gene summary	The protein encoded by this gene is a heat stable, nuclear protein and functions as a molecular chaperone. It is thought to regulate dimerization, DNA binding, and transcriptional activity of basic region-leucine zipper (bZIP) proteins. [provided by RefSeq, Jul 2008]
Chromosome	17
Cytoband	q25.3
Chromosome location (bp)	81887844 - 81891586
Protein evidence	Evidence at protein level (all genes)
Ensembl	ENSG00000183684 (version 83.38)
Entrez gene	10189
UniProt
neXtProt
Antibodypedia	ALYREF antibodies

PROTEIN BROWSER

ALYREF-001

PROTEIN INFORMATION
Splice variant	UniProt	Protein class	Gene ontology	Length & mass	Signal peptide (predicted)	Transmembrane regions (predicted)
ALYREF-001 ENSP00000421592 ENST00000505490	E9PB61 [Direct mapping] THO complex subunit 4 Show all	Predicted intracellular proteins Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0000166 [nucleotide binding] GO:0003676 [nucleic acid binding] GO:0005634 [nucleus] GO:0005654 [nucleoplasm] GO:0043231 [intracellular membrane-bounded organelle] Show all	264 aa 27.6 kDa	No	0

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.