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ANTIBODY INFORMATION
 
 

Antibody HPA042562

  

Antibody HPA055695

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA042562   HPA055695  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 9   12  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA042562

  

Antibody HPA055695

  
Immunocytochemistry


Validated
  

Validated

Validated
  
Immunohistochemistry

Supported    
Western blot

Supported   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA042562

  

Antibody HPA055695

  

Formal validation: Genetic

  
Validated

Significant downregulation > 25% by one siRNA.		  

 
 

Relative fluorescence intensity (RFI)

  

Figure description

   Nuclear region of segmented cells in 10x-images  

Antibody dilution

   1:92  
 

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA055695.		  
Validated

Antibody staining overlaps with antibody HPA042562.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to nucleus & nucleoli.   Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli.  

Antibody dilution

 1:57   1:92  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA042562

  

Antibody HPA055695

  

Standard validation

 Supported    

   

Figure description

 Immunohistochemical staining of human cerebellum shows strong nucleolar positivity in Purkinje cells.    

Expression

 RNA: detected in 34 tissues
Protein: detected in 42 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:200    

Literature conformity

 Consistent with extensive gene/protein characterization data.    

RNA consistency

 Mainly consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA042562

  

Antibody HPA055695

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 89, 77   89  

Antibody dilution

 1:250   1:300  

PROTEIN ARRAY
 
 

Antibody HPA042562

  

Antibody HPA055695

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3650  

ANTIGEN INFORMATION
 
 

Antibody HPA042562

  

Antibody HPA055695

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 109   113  

Antigen sequence

 
LWKDVTEAEQAEEQARQEEQVVRQQELDNLLHEKRYLRALGLAISLDRPH
TVLTVIQAIRRDPEACEKLEATMLRLRRDQKEALLRFCVTWNTNSRHCHE
AQAVLGVLL
  
RFKTNYAVERKIEPFYKGGKAQLDQTGQHLFCVCGTRVNILEVASGAVLR
SLEQEDQEDITAFDLSPDNEVLVTASRALLLAQWAWQEGSVTRLWKAIHT
APVATMAFDPTST
 

Matching transcripts

 TBL3-001 - ENSP00000454836 [100%]
TBL3-004 - ENSP00000331815 [100%]
  TBL3-001 - ENSP00000454836 [100%]
 

Other gene match

    
ANTIGEN VIEW
TBL3-001
TBL3-004
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.