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ARSI

ANTIBODY INFORMATION
 
 

Antibody HPA038386

  

Antibody HPA038398

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA038386   HPA038398  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 7   14  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA038386

  

Antibody HPA038398

  
Immunocytochemistry

Uncertain    
Immunohistochemistry

Uncertain   Uncertain  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA038386

  

Antibody HPA038398

  

Standard validation

 Uncertain    

   

Figure description

 Immunofluorescent staining of human cell line Hep G2 shows localization to the Golgi apparatus.    

Antibody dilution

 1:6    

Literature conformity

 The subcellular location is not consistent with literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA038386

  

Antibody HPA038398

  

Standard validation

 Uncertain   Uncertain  

   

Figure description

 Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.   Immunohistochemical staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.  

Expression

 RNA: detected in 15 tissues
Protein: detected in 14 cell types		   RNA: detected in 15 tissues
Protein: detected in 21 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:10   1:500  

Literature conformity

 Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.  

RNA consistency

 Not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA038386

  

Antibody HPA038398

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 64, 48.3   64, 48.3  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA038386

  

Antibody HPA038398

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA038386

  

Antibody HPA038398

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 81   63  

Antigen sequence

 
TYDNCDGPGVCGFDLHEGENVAWGLSGQYSTMLYAQRASHILASHSPQRP
LFLYVAFQAVHTPLQSPREYLYRYRTMGNVA
  
SADPYEREDLAGQRPDVVRTLLARLAEYNRTAIPVRYPAENPRAHPDFNG
GAWGPWASDEEEE
 

Matching transcripts

 ARSI-001 - ENSP00000333395 [100%]
ARSI-002 - ENSP00000426879 [100%]
  ARSI-001 - ENSP00000333395 [100%]
ARSI-002 - ENSP00000426879 [100%]
 

Other gene match

    
ANTIGEN VIEW
ARSI-001
ARSI-002
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.