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Immunofluorescent staining of human cell line SH-SY5Y shows localization to nucleoplasm.
Antibody dilution
1:72
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA001403
Standard validation
Supported
Figure description
Immunohistochemical staining of human pituitary gland shows moderate nuclear positivity in anterior cells.
Expression
RNA: detected in 0 tissues Protein: detected in 30 cell types
Retrieval
HIER pH6
Antibody dilution
1:1200
Literature conformity
Consistent with extensive gene/protein characterization data.
RNA consistency
No internal RNA expression data available for correlation.
WESTERN BLOT
Antibody HPA001403
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY416023)
Target mass (kDa)
27.7
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA001403
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody dilution
1:3000
ANTIGEN INFORMATION
Antibody HPA001403
Antigen
Recombinant protein fragment
Length (aa)
151
Antigen sequence
YQEAEKLRGRPLGPVDKYRVRKKFPLPRTIWDGEQKTHCFKERTRNLLRE
WYLQDPYPNPSKKRELAQATGLTPTQVGNWFKNRRQRDRAAAAKNRLQQQ
VLSQGSGRALRAEGDGTPEVLGVATSPAASLSSKAATSAISITSSDSECD
I
