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SLITRK6

ANTIBODY INFORMATION
 
 

Antibody HPA014491

  

Antibody HPA014513

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA014491   HPA014513  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 6   16  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA014491

  

Antibody HPA014513

  
Immunocytochemistry

  Supported  
Immunohistochemistry

Uncertain    
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA014491

  

Antibody HPA014513

  

Standard validation

   Supported  

   

Figure description

   Immunofluorescent staining of human cell line RT4 shows localization to plasma membrane.  

Antibody dilution

   1:159  

Literature conformity

   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA014491

  

Antibody HPA014513

  

Standard validation

 Uncertain    

   

Figure description

 Immunohistochemical staining of human urinary bladder shows moderate cytoplasmic and membranous positivity in urothelial cells.    

Expression

 RNA: detected in 20 tissues
Protein: detected in 70 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:175    

Literature conformity

 No avaliable gene/protein characterization data.    

RNA consistency

 Not consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA014491

  

Antibody HPA014513

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

    

Target mass (kDa)

 95.1   95.1  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA014491

  

Antibody HPA014513

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA014491

  

Antibody HPA014513

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 147   147  

Antigen sequence

 
HHTTERPSASLYEQHMVSPMVHVYRSPSFGPKHLEEEEERNEKEGSDAKH
LQRSLLEQENHSPLTGSNMKYKTTNQSTEFLSFQDASSLYRNILEKEREL
QQLGITEYLRKNIAQLQPDMEAHYPGAHEELKLMETLMYSRPRKVLV
  
HHTTERPSASLYEQHMVSPMVHVYRSPSFGPKHLEEEEERNEKEGSDAKH
LQRSLLEQENHSPLTGSNMKYKTTNQSTEFLSFQDASSLYRNILEKEREL
QQLGITEYLRKNIAQLQPDMEAHYPGAHEELKLMETLMYSRPRKVLV
 

Matching transcripts

 SLITRK6-001 - ENSP00000383143 [100%]
  SLITRK6-001 - ENSP00000383143 [100%]
 

Other gene match

    
ANTIGEN VIEW
SLITRK6-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.