ANKFY1 - Antibodies - The Human Protein Atlas

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ANKFY1

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA024513

Antibody HPA024522

Antibody HPA065849

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA024513

HPA024522

HPA065849

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA024513

Antibody HPA024522

Antibody HPA065849

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA024513

Antibody HPA024522

Antibody HPA065849

Formal validation: Independent

Validated

Antibody staining overlaps with antibody HPA065849.

Validated

Antibody staining overlaps with antibody HPA024522.

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.

Immunofluorescent staining of human cell line A549 shows localization to endosomes.

Antibody dilution

1:35

1:95

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA024513

Antibody HPA024522

Antibody HPA065849

Standard validation

Approved

Figure description

Immunohistochemical staining of human kidney shows strong membranous positivity in tubular cells.

Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 28 cell types

RNA: detected in 37 tissues
Protein: detected in 48 cell types

Retrieval

HIER pH6

Antibody dilution

1:200

1:10

Literature conformity

Partly consistent with gene/protein characterization data.

Consistent with gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA024513

Antibody HPA024522

Antibody HPA065849

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

132.8, 128.5, 128.4

Antibody dilution

1:250

1:320

PROTEIN ARRAY

Antibody HPA024513

Antibody HPA024522

Antibody HPA065849

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:500

1:3000

1:3850

ANTIGEN INFORMATION

Antibody HPA024513

Antibody HPA024522

Antibody HPA065849

Antigen

Recombinant protein fragment

Length (aa)

100

106

Antigen sequence

YVFSQLLQCKQLDLELKDHEGSTALWLAVQHITVSSDQSVNPFEDVPVVN
GTSFDENSFAARLIQRGSHTDAPDTATGNCLLQRAAGAGNEAAALFLATN

SPLHILGQYGKENAAAIFDLFLECMPGYPLDKPDADGSTVLLLAYMKGNA
NLCRAIVRSGARLGVNNNQGVNIFNYQVATKQLLFRLLDMLSKEPPWCDG
SYCYEC

ICTRGADMSVPDEKGNPPLWLALANNLEDIASTLVRHGCDATCWGPGPGG
CLQTLLHRAIDENNEPTACFLIRSGCDVNSPR

Matching transcripts

ANKFY1-001 - ENSP00000459943 [100%]
ANKFY1-003 - ENSP00000459775 [100%]
ANKFY1-008 - ENSP00000343362 [100%]

Other gene match

ANTIGEN VIEW

ANKFY1-001	ANKFY1-003	ANKFY1-008

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.