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Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.
Immunofluorescent staining of human cell line RT4 shows localization to plasma membrane.
Antibody dilution
1:159
1:88
Literature conformity
The subcellular location is supported by literature.
The subcellular location is not consistent with literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA042707
Antibody HPA043622
Formal validation: Independent
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Spearman correlation with HPA043622 across 72 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA042707 across 72 cell types.
Standard validation
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human testis shows nuclear and cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows nuclear and cytoplasmic positivity in cells in seminiferous ducts.
Expression
RNA: detected in 37 tissues Protein: detected in 75 cell types
RNA: detected in 37 tissues Protein: detected in 64 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:250
1:150
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA042707
Antibody HPA043622
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
210.8, 194.9
210.8, 194.9
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA042707
Antibody HPA043622
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
