We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.
Immunohistochemical staining of human tonsil shows strong cytoplasmic and nuclear positivity.
Immunohistochemical staining of human tonsil shows strong cytoplasmic and nuclear positivity in lymphoid cells outside reaction centra.
Immunohistochemical staining of human tonsil shows strong nuclear and cytoplasmic positivity in germinal center cells and non-germinal center cells.
Immunohistochemical staining of human spleen shows strong cytoplasmic and nuclear positivity in cells in red pulp.
Immunohistochemical staining of human tonsil shows moderate cytoplasmic positivity in non-germinal center cells.
Immunohistochemical staining of human spleen shows distinct positivity in cells in red pulp.
Expression
RNA: detected in 37 tissues Protein: detected in 52 cell types
RNA: detected in 37 tissues Protein: detected in 23 cell types
RNA: detected in 37 tissues Protein: detected in 30 cell types
RNA: detected in 37 tissues Protein: detected in 21 cell types
RNA: detected in 37 tissues Protein: detected in 21 cell types
RNA: detected in 37 tissues Protein: detected in 19 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:250
1:400
1:3500
1:3000
1:8000
1:16000
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Consistent with RNA expression data.
Consistent with RNA expression data.
Consistent with RNA expression data.
Consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA007973
Antibody CAB002618
Antibody CAB027387
Antibody CAB058698
Antibody CAB068227
Antibody CAB068228
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY418991)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
11.7
11.7
11.7
11.7
11.7
11.7
Antibody dilution
1:250
1:500
1:500
1:500
1:1000
1:1000
PROTEIN ARRAY
Antibody HPA007973
Antibody CAB002618
Antibody CAB027387
Antibody CAB058698
Antibody CAB068227
Antibody CAB068228
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody dilution
1:500
ANTIGEN INFORMATION
Antibody HPA007973
Antibody CAB002618
Antibody CAB027387
Antibody CAB058698
Antibody CAB068227
Antibody CAB068228
Antigen
Recombinant protein fragment
Not known
Recombinant protein
Recombinant protein
Recombinant protein
Recombinant protein
Length (aa)
101
Antigen sequence
MACPLEKALDVMVSTFHKYSGKEGDKFKLNKSELKELLTRELPSFLGKRT
DEAAFQKLMSNLDSNRDNEVDFQEYCVFLSCIAMMCNEFFEGFPDKQPRK
K
