SERPINA1 - Antibodies - The Human Protein Atlas

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SERPINA1

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA000927

Antibody HPA001292

Antibody CAB013211

Antibody CAB016648

Antibody CAB073396

Provider

Atlas Antibodies
Sigma-Aldrich

Zymed

AbFrontier

NCI-CPTC

Product name

HPA000927

HPA001292

18-0002

LF-MA0175

CPTC-SERPINA1-1

Host species

Rabbit

Mouse

Clonality

pAb

mAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Not known

Ammonium Sulfate Precipitation

Protein A/G

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA000927

Antibody HPA001292

Antibody CAB013211

Antibody CAB016648

Antibody CAB073396

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA000927

Antibody HPA001292

Antibody CAB013211

Antibody CAB016648

Antibody CAB073396

Formal validation: Independent

Validated

Antibody staining overlaps with antibody HPA001292.

Validated

Antibody staining overlaps with antibody HPA000927.

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line Hep G2 shows localization to vesicles.

Antibody dilution

1:65

1:10

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA000927

Antibody HPA001292

Antibody CAB013211

Antibody CAB016648

Antibody CAB073396

Formal validation: Orthogonal

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.

Standard validation

Supported

Figure description

Immunohistochemical staining of human kidney shows positivity in plasma and cells in tubules.

Immunohistochemical staining of human kidney shows positivity of plasma.

Immunohistochemical staining of human stomach shows secreted positivity in glandular cells.

Expression

RNA: detected in 36 tissues
Protein: detected in 18 cell types

RNA: detected in 36 tissues
Protein: detected in 11 cell types

RNA: detected in 36 tissues
Protein: detected in 28 cell types

RNA: detected in 36 tissues
Protein: detected in 22 cell types

RNA: detected in 36 tissues
Protein: detected in 40 cell types

Retrieval

HIER pH6

Antibody dilution

1:4000

1:1000

1:100

1:30000

1:18000

Literature conformity

Consistent with extensive gene/protein characterization data.

Partly consistent with extensive gene/protein characterization data.

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA000927

Antibody HPA001292

Antibody CAB013211

Antibody CAB016648

Antibody CAB073396

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.

Figure description

Lane 1: Marker [kDa] 219, 112, 85, 49, 32, 25, 17.5
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 17.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 213, 118, 83, 50, 33, 26, 18
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

46.7, 40.3

46.7

46.7, 40.3, 17.1, 15.7, 12.7, 11.8, 9.9, 2.2

Antibody dilution

1:500

1:250

1:500

PROTEIN ARRAY

Antibody HPA000927

Antibody HPA001292

Antibody CAB013211

Antibody CAB016648

Antibody CAB073396

Standard validation

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:1000

1:500

ANTIGEN INFORMATION

Antibody HPA000927

Antibody HPA001292

Antibody CAB013211

Antibody CAB016648

Antibody CAB073396

Antigen

Recombinant protein fragment

Not known

Native protein

Recombinant protein

Length (aa)

150

155

Antigen sequence

HDQDHPTFNKITPNLAEFAFSLYRQLAHQSNSTNIFFSPVSIATAFAMLS
LGTKADTHDEILEGLNFNLTEIPEAQIHEGFQELLRTLNQPDSQLQLTTG
NGLFLSEGLKLVDKFLEDVKKLYHSEAFTVNFGDTEEAKKQINDYGAPHR

GMFNIQHCKKLSSWVLLMKYLGNATAIFFLPDEGKLQHLENELTHDIITK
FLENEDRRSASLHLPKLSITGTYDLKSVLGQLGITKVFSNGADLSGVTEE
APLKLSKAVHKAVLTIDEKGTEAAGAMFLEAIPMSIPPEVKFNKPFVFLM
GAPHR

Matching transcripts