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ARHGEF15

ANTIBODY INFORMATION
 
 

Antibody HPA044443

  

Antibody HPA063495

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA044443   HPA063495  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 9   16  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA044443

  

Antibody HPA063495

  
Immunocytochemistry

Approved   Approved  
Immunohistochemistry

Uncertain    
Western blot

Uncertain   Uncertain  
Protein array

Approved   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA044443

  

Antibody HPA063495

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunofluorescent staining of human cell line HUVEC TERT2 shows localization to plasma membrane.   Immunofluorescent staining of human cell line HUVEC TERT2 shows localization to nucleoplasm & plasma membrane.  

Antibody dilution

 1:200   1:106  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA044443

  

Antibody HPA063495

  

Standard validation

 Uncertain    

   

Figure description

 Immunohistochemical staining of human stomach shows strong cytoplasmic and membranous positivity in glandular cells.    

Expression

 RNA: detected in 35 tissues
Protein: detected in 69 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:200    

Literature conformity

 No avaliable gene/protein characterization data.    

RNA consistency

 Not consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA044443

  

Antibody HPA063495

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

   

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 91.9   91.9, 21.1  

Antibody dilution

 1:250   1:360  

PROTEIN ARRAY
 
 

Antibody HPA044443

  

Antibody HPA063495

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:6000   1:4250  

ANTIGEN INFORMATION
 
 

Antibody HPA044443

  

Antibody HPA063495

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 95   80  

Antigen sequence

 
PTHRLLQASSLSDMQRWLGAFPTPGPLPCSPDTIYEDCDCSQELCSESSA
PAKTEGRSLESRAAPKHLHKTPEGWLKGLPGAFPAQLVCEVTGEH
  
PSGSPCTPLLPMAGVLAQNGSASAPGTVRRLAGRFEGGAEGRAQDADAPE
PGLQARADVNGEREAPLTGSGSQENGAPDA
 

Matching transcripts

 ARHGEF15-001 - ENSP00000355026 [100%]
ARHGEF15-201 - ENSP00000412505 [100%]
  ARHGEF15-001 - ENSP00000355026 [100%]
ARHGEF15-004 - ENSP00000464540 [100%]
ARHGEF15-201 - ENSP00000412505 [100%]
 

Other gene match

    
ANTIGEN VIEW
ARHGEF15-001
ARHGEF15-004
ARHGEF15-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.