Fields »
ABOUT
HELP
BLOG
TCEB3B

ANTIBODY INFORMATION
 
 

Antibody HPA041009

  

Antibody HPA043501

  

Antibody HPA048971

  

Antibody HPA049178

  

Antibody HPA050086

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA041009   HPA043501   HPA048971   HPA049178   HPA050086  

Host species

 Rabbit   Rabbit   Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

     TCEB3C - ENSG00000183791 [100%]
TCEB3CL2 - ENSG00000274744 [100%]
TCEB3CL - ENSG00000275553 [100%]
RP11-49K24.9 - ENSG00000278674 [100%]
   TCEB3C - ENSG00000183791 [100%]
TCEB3CL2 - ENSG00000274744 [98%]
TCEB3CL - ENSG00000275553 [100%]
RP11-49K24.9 - ENSG00000278674 [100%]
   TCEB3C - ENSG00000183791 [100%]
TCEB3CL2 - ENSG00000274744 [100%]
TCEB3CL - ENSG00000275553 [100%]
RP11-49K24.9 - ENSG00000278674 [100%]
  

Released in version

 9   9   12   12   12  

References

          

VALIDATION SUMMARY
 
 

Antibody HPA041009

  

Antibody HPA043501

  

Antibody HPA048971

  

Antibody HPA049178

  

Antibody HPA050086

  
Immunohistochemistry

Approved   Approved   Approved   Approved   Approved  
Western blot

Uncertain   Uncertain   Uncertain   Uncertain   Uncertain  
Protein array

Supported   Supported   Approved   Approved   Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA041009

  

Antibody HPA043501

  

Antibody HPA048971

  

Antibody HPA049178

  

Antibody HPA050086

  

Standard validation

 Approved   Approved   Approved   Approved   Approved  

         

Figure description

 Immunohistochemical staining of human liver shows moderate cytoplasmic positivity in hepatocytes.   Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic and nuclear positivity in astrocytes.   Immunohistochemical staining of human adrenal gland shows strong cytoplasmic and membranous positivity in glandular cells.   Immunohistochemical staining of human stomach, lower shows moderate cytoplasmic and nuclear positivity in glandular cells.   Immunohistochemical staining of human testis shows moderate nucleolar positivity in cells in seminiferus ducts and Leydig cells.  

Expression

 RNA: detected in 1 tissues
Protein: detected in 12 cell types		   RNA: detected in 1 tissues
Protein: detected in 64 cell types		   RNA: detected in 1 tissues
Protein: detected in 7 cell types		   RNA: detected in 1 tissues
Protein: detected in 52 cell types		   RNA: detected in 1 tissues
Protein: detected in 34 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:225   1:700   1:100   1:10   1:175  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Not consistent with RNA expression data.   Not consistent with RNA expression data.   Not consistent with RNA expression data.   Not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA041009

  

Antibody HPA043501

  

Antibody HPA048971

  

Antibody HPA049178

  

Antibody HPA050086

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

         

Figure description

          

Target mass (kDa)

 83.9   83.9   83.9, 60.7, 59.8, 59.7   83.9, 60.7, 59.8, 59.7, 43.9   83.9, 60.7, 59.8, 59.7, 43.9  

Antibody dilution

 1:250   1:250   1:250   1:180   1:350  

PROTEIN ARRAY
 
 

Antibody HPA041009

  

Antibody HPA043501

  

Antibody HPA048971

  

Antibody HPA049178

  

Antibody HPA050086

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

         

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:6000   1:5850   1:1450   1:2800  

ANTIGEN INFORMATION
 
 

Antibody HPA041009

  

Antibody HPA043501

  

Antibody HPA048971

  

Antibody HPA049178

  

Antibody HPA050086

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 80   80   25   47   26  

Antigen sequence

 
GHKSSRQEKRPLCAQGDWHSPTLIREKSCGACLREETPRMPSWASARDRQ
PSDFKTDKEGGQAGSGQRVPALEEAPDSHQ
  
QKRPQHSHSNKKRPSLDGRDPGNGTHGLSPEEKEQLSNDRETQEGKPPTA
HLDRTSVSSLSEVEEVDMAEEFEQPTLSCE
  
PSHSPEHRRTARRTPPGQQRPHPRS
  
KSPPVQESQSERLQAAGADSAGPKTVPSHVFSELWDPSEAWMQANYD
  
KTRKQAAKKVAPLMAKAIRDYKGRFS
 

Matching transcripts

 TCEB3B-001 - ENSP00000331302 [100%]
  TCEB3B-001 - ENSP00000331302 [100%]
  TCEB3B-001 - ENSP00000331302 [100%]
TCEB3B-201 - ENSP00000479491 [100%]
  TCEB3B-001 - ENSP00000331302 [96%]
TCEB3B-201 - ENSP00000479491 [96%]
  TCEB3B-001 - ENSP00000331302 [96%]
TCEB3B-201 - ENSP00000479491 [96%]
 

Other gene match

     TCEB3C - ENSG00000183791 [100%]
TCEB3CL2 - ENSG00000274744 [100%]
TCEB3CL - ENSG00000275553 [100%]
RP11-49K24.9 - ENSG00000278674 [100%]
   TCEB3C - ENSG00000183791 [100%]
TCEB3CL2 - ENSG00000274744 [98%]
TCEB3CL - ENSG00000275553 [100%]
RP11-49K24.9 - ENSG00000278674 [100%]
   TCEB3C - ENSG00000183791 [100%]
TCEB3CL2 - ENSG00000274744 [100%]
TCEB3CL - ENSG00000275553 [100%]
RP11-49K24.9 - ENSG00000278674 [100%]
  
ANTIGEN VIEW
TCEB3B-001
TCEB3B-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.