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Immunohistochemical staining of human testis shows strong cytoplasmic and nuclear positivity in cells in seminiferus ducts, Leydig cells showed moderate nuclear staining.
Expression
RNA: detected in 37 tissues Protein: detected in 29 cell types
Retrieval
HIER pH6
Antibody dilution
1:100
Literature conformity
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA059561
Standard validation
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
26.2, 17.3
Antibody dilution
1:110
PROTEIN ARRAY
Antibody HPA059561
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody dilution
1:1150
ANTIGEN INFORMATION
Antibody HPA059561
Antigen
Recombinant protein fragment
Length (aa)
51
Antigen sequence
MSVSEIFVELQGFLAAEQDIREEIRKVVQSLEQTAREILTLLQGVHQGAG
F
