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Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus.
Antibody dilution
1:200
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA054058
Standard validation
Approved
Figure description
Immunohistochemical staining of human nasopharynx shows strong cytoplasmic and nuclear positivity in respiratory epithelial cells.
Expression
RNA: detected in 37 tissues Protein: detected in 76 cell types
Retrieval
HIER pH6
Antibody dilution
1:100
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA054058
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY404118)
Target mass (kDa)
18.8, 11.1, 11
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA054058
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
