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AS3MT

ANTIBODY INFORMATION
 
 

Antibody HPA017856

  

Antibody HPA027708

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA017856   HPA027708  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 4   6  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA017856

  

Antibody HPA027708

  
Immunohistochemistry


Validated
  

Validated
  
Western blot

Supported   Uncertain  
Protein array

Supported   Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA017856

  

Antibody HPA027708

  

Formal validation: Independent
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  

 

  
 

  

Figure description

 Distribution of protein expression (antibody staining).
Spearman correlation with HPA027708 across 70 cell types.		   Distribution of protein expression (antibody staining).
Spearman correlation with HPA017856 across 70 cell types.		  
 

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human small intestine shows cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 36 tissues
Protein: detected in 57 cell types		   RNA: detected in 36 tissues
Protein: detected in 65 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:35   1:10  

Literature conformity

 Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA017856

  

Antibody HPA027708

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 41.7, 38   41.7, 38  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA017856

  

Antibody HPA027708

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500   1:500  

ANTIGEN INFORMATION
 
 

Antibody HPA017856

  

Antibody HPA027708

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 145   116  

Antigen sequence

 
VLQEAYRVLKHGGELYFSDVYTSLELPEEIRTHKVLWGECLGGALYWKEL
AVLAQKIGFCPPRLVTANLITIQNKELERVIGDCRFVSATFRLFKHSKTG
PTKRCQVIYNGGITGHEKELMFDANFTFKEGEIVEVDEETAAILK
  
REALQNVHEEVALRYYGCGLVIPEHLENCWILDLGSGSGRDCYVLSQLVG
EKGHVTGIDMTKGQVEVAEKYLDYHMEKYGFQASNVTFIHGYIEKLGEAG
IKNESHDIVVSNCVIN
 

Matching transcripts

 AS3MT-001 - ENSP00000358896 [100%]
AS3MT-201 - ENSP00000479361 [100%]
  AS3MT-001 - ENSP00000358896 [100%]
AS3MT-201 - ENSP00000479361 [100%]
 

Other gene match

    
ANTIGEN VIEW
AS3MT-001
AS3MT-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.