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PPME1

ANTIBODY INFORMATION
 
 

Antibody HPA043900

  

Antibody HPA064396

  

Antibody CAB004541

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA043900   HPA064396   sc-25278  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 13   14   2  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA043900

  

Antibody HPA064396

  

Antibody CAB004541

  
Immunocytochemistry

  Approved   Approved  
Immunohistochemistry

Uncertain     Uncertain  
Western blot

Uncertain   Supported   Supported  
Protein array

Approved   Approved    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA043900

  

Antibody HPA064396

  

Antibody CAB004541

  

Standard validation

   Approved   Approved  

     

Figure description

   Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.   Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus.  

Antibody dilution

   1:200   1:2000  

Literature conformity

   The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA043900

  

Antibody HPA064396

  

Antibody CAB004541

  

Standard validation

 Uncertain     Uncertain  

     

Figure description

 Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.     Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferus ducts.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 54 cell types		     RNA: detected in 37 tissues
Protein: detected in 67 cell types		  

Retrieval

 HIER pH6     HIER pH6  

Antibody dilution

 1:300     1:3000  

Literature conformity

 No avaliable gene/protein characterization data.     No avaliable gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.     Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA043900

  

Antibody HPA064396

  

Antibody CAB004541

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

     

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 43.9, 42.3   43.9, 42.3   43.9, 42.3, 15.1  

Antibody dilution

 1:250   1:1050   1:500  

PROTEIN ARRAY
 
 

Antibody HPA043900

  

Antibody HPA064396

  

Antibody CAB004541

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:12550    

ANTIGEN INFORMATION
 
 

Antibody HPA043900

  

Antibody HPA064396

  

Antibody CAB004541

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 70   99    

Antigen sequence

 
PPLPGSGGSQSGAKMRMGPGRKRDFSPVPWSQYFESMEDVEVENETGKDT
FRVYKSGSEGPVLLLLHGGG
  
AIAVHTASSNLVPSLLGLCMIDVVEGTAMDALNSMQNFLRGRPKTFKSLE
NAIEWSVKSGQIRNLESARVSMVGQVKQCEGITSPEGSKSIVEGIIEEE
  

											 
																				

																		

										
											
Matching transcripts

												
																					PPME1-001 - ENSP00000329867 [100%] 
PPME1-002 - ENSP00000381461 [100%] 

											 
																						PPME1-001 - ENSP00000329867 [100%] 
PPME1-002 - ENSP00000381461 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
PPME1-001
PPME1-002
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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