DDOST - Antibodies - The Human Protein Atlas

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DDOST

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA046841

Antibody HPA052867

Antibody CAB009746

Provider

Atlas Antibodies
Sigma-Aldrich

Santa Cruz Biotechnology

Product name

HPA046841

HPA052867

sc-25558

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Protein A/G

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA046841

Antibody HPA052867

Antibody CAB009746

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA046841

Antibody HPA052867

Antibody CAB009746

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.

Antibody dilution

1:49

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA046841

Antibody HPA052867

Antibody CAB009746

Formal validation: Independent

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Pearson correlation with HPA052867 across 46 cell lines.

Distribution of protein expression (antibody staining).
Pearson correlation with HPA046841 across 46 cell lines.

Formal validation: Independent

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Spearman correlation with HPA052867 across 74 cell types.

Distribution of protein expression (antibody staining).
Spearman correlation with HPA046841 across 74 cell types.

Standard validation

Supported

Figure description

Immunohistochemical staining of human duodenum shows cytoplasmic positivity in leukocytes and glandular cells.

Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.

Immunohistochemical staining of human duodenum shows cytoplasmic positivity in glandular cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 54 cell types

RNA: detected in 37 tissues
Protein: detected in 52 cell types

RNA: detected in 37 tissues
Protein: detected in 79 cell types

Retrieval

HIER pH6

Antibody dilution

1:1000

1:1500

1:15

Literature conformity

Consistent with gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA046841

Antibody HPA052867

Antibody CAB009746

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 219, 111, 83, 48, 32, 26, 17
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

50.8, 48.8

50.8, 48.8, 17.2

Antibody dilution

1:250

1:700

1:500

PROTEIN ARRAY

Antibody HPA046841

Antibody HPA052867

Antibody CAB009746

Standard validation

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:1000

1:5600

ANTIGEN INFORMATION

Antibody HPA046841

Antibody HPA052867

Antibody CAB009746

Antigen

Recombinant protein fragment

Recombinant protein

Length (aa)

131

145

Antigen sequence

NYELAVALSRWVFKEEGVLRVGPVSHHRVGETAPPNAYTVTDLVEYSIVI
QQLSNGKWVPFDGDDIQLEFVRIDPFVRTFLKKKGGKYSVQFKLPDVYGV
FQFKVDYNRLGYTHLYSSTQVSVRPLQHTQY

LVLLDNLNVRETHSLFFRSLKDRGFELTFKTADDPSLSLIKYGEFLYDNL
IIFSPSVEDFGGNINVETISAFIDGGGSVLVAASSDIGDPLRELGSECGI
EFDEEKTAVIDHHNYDISDLGQHTLIVADTENLLKAPTIVGKSSL

Matching transcripts

DDOST-001 - ENSP00000364188 [100%]
DDOST-006 - ENSP00000473655 [100%]
DDOST-201 - ENSP00000399457 [100%]

DDOST-001 - ENSP00000364188 [100%]
DDOST-006 - ENSP00000473655 [100%]
DDOST-201 - ENSP00000399457 [100%]
DDOST-005 - ENSP00000475634 [80%]

Other gene match

ANTIGEN VIEW

DDOST-001	DDOST-005	DDOST-006	DDOST-201

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.