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TUBA3D

ANTIBODY INFORMATION
 
 

Antibody HPA039247

  

Antibody HPA043684

  

Antibody HPA063394

  

Antibody CAB004972

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Abnova
  

Product name

 HPA039247   HPA043684   HPA063394   H00007278-M01  

Host species

 Rabbit   Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

 TUBA1B - ENSG00000123416 [100%]
TUBA4A - ENSG00000127824 [96%]
TUBA3E - ENSG00000152086 [100%]
TUBA1A - ENSG00000167552 [100%]
TUBA1C - ENSG00000167553 [100%]
TUBA3C - ENSG00000198033 [100%]
   TUBA1B - ENSG00000123416 [100%]
TUBA4A - ENSG00000127824 [100%]
TUBA3E - ENSG00000152086 [100%]
TUBA1A - ENSG00000167552 [100%]
TUBA1C - ENSG00000167553 [96%]
TUBA8 - ENSG00000183785 [88%]
TUBA3C - ENSG00000198033 [100%]
   TUBA1B - ENSG00000123416 [100%]
TUBA4A - ENSG00000127824 [100%]
TUBA3E - ENSG00000152086 [93%]
TUBA1A - ENSG00000167552 [100%]
TUBA1C - ENSG00000167553 [97%]
TUBAL3 - ENSG00000178462 [93%]
TUBA8 - ENSG00000183785 [100%]
TUBA3C - ENSG00000198033 [100%]
    

Released in version

 10   10   13   2  

References

        

VALIDATION SUMMARY
 
 

Antibody HPA039247

  

Antibody HPA043684

  

Antibody HPA063394

  

Antibody CAB004972

  
Immunocytochemistry

Supported   Supported      
Immunohistochemistry

Uncertain   Uncertain   Uncertain   Uncertain  
Western blot

Supported   Supported   Supported   Uncertain  
Protein array

Supported   Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA039247

  

Antibody HPA043684

  

Antibody HPA063394

  

Antibody CAB004972

  

Standard validation

 Supported   Supported      

       

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to microtubules.   Immunofluorescent staining of human cell line U-2 OS shows localization to microtubules.      

Antibody dilution

 1:7   1:75      

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA039247

  

Antibody HPA043684

  

Antibody HPA063394

  

Antibody CAB004972

  

Standard validation

 Uncertain   Uncertain   Uncertain   Uncertain  

       

Figure description

 Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells of the seminiferous ducts.   Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in cells of the seminiferous ducts.   Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.   Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.  

Expression

 RNA: detected in 8 tissues
Protein: detected in 79 cell types		   RNA: detected in 8 tissues
Protein: detected in 41 cell types		   RNA: detected in 8 tissues
Protein: detected in 71 cell types		   RNA: detected in 8 tissues
Protein: detected in 75 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:600   1:150   1:40   1:2500  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Not consistent with RNA expression data.   Not consistent with RNA expression data.   Not consistent with RNA expression data.   Not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA039247

  

Antibody HPA043684

  

Antibody HPA063394

  

Antibody CAB004972

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

       

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 57.7, 50.2, 50.1, 50, 49.9, 48.3, 46.1, 27.6, 24.8, 21.7, 19.8, 16.9, 14.4, 13.2   57.7, 52, 50.2, 50.1, 50, 49.9, 48.3, 46.3, 46.1, 43, 31, 17.7   57.7, 52, 50.2, 50.1, 50, 49.9, 48.3, 46.3, 45.5, 43, 31   50  

Antibody dilution

 1:250   1:250   1:80   1:500  

PROTEIN ARRAY
 
 

Antibody HPA039247

  

Antibody HPA043684

  

Antibody HPA063394

  

Antibody CAB004972

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

       

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:500   1:3000   1:500    

ANTIGEN INFORMATION
 
 

Antibody HPA039247

  

Antibody HPA043684

  

Antibody HPA063394

  

Antibody CAB004972

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 28   24   29    

Antigen sequence

 
LEHGIQPDGQMPSDKTIGGGDDSFNTFF
  
ATYAPVISAEKAYHEQLSVAEITN
  
NTTAIAEAWARLDHKFDLMYAKRAFVHWY
   

Matching transcripts

 TUBA3D-001 - ENSP00000326042 [100%]
  TUBA3D-001 - ENSP00000326042 [100%]
  TUBA3D-001 - ENSP00000326042 [100%]
   

Other gene match

 TUBA1B - ENSG00000123416 [100%]
TUBA4A - ENSG00000127824 [96%]
TUBA3E - ENSG00000152086 [100%]
TUBA1A - ENSG00000167552 [100%]
TUBA1C - ENSG00000167553 [100%]
TUBA3C - ENSG00000198033 [100%]
   TUBA1B - ENSG00000123416 [100%]
TUBA4A - ENSG00000127824 [100%]
TUBA3E - ENSG00000152086 [100%]
TUBA1A - ENSG00000167552 [100%]
TUBA1C - ENSG00000167553 [96%]
TUBA8 - ENSG00000183785 [88%]
TUBA3C - ENSG00000198033 [100%]
   TUBA1B - ENSG00000123416 [100%]
TUBA4A - ENSG00000127824 [100%]
TUBA3E - ENSG00000152086 [93%]
TUBA1A - ENSG00000167552 [100%]
TUBA1C - ENSG00000167553 [97%]
TUBAL3 - ENSG00000178462 [93%]
TUBA8 - ENSG00000183785 [100%]
TUBA3C - ENSG00000198033 [100%]
    
ANTIGEN VIEW
TUBA3D-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.