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TUBA8

ANTIBODY INFORMATION
 
 

Antibody HPA043684

  

Antibody HPA063394

  

Antibody CAB046031

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Origene
  

Product name

 HPA043684   HPA063394   TA501096  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

 TUBA3D - ENSG00000075886 [100%]
TUBA1B - ENSG00000123416 [100%]
TUBA4A - ENSG00000127824 [100%]
TUBA3E - ENSG00000152086 [100%]
TUBA1A - ENSG00000167552 [100%]
TUBA1C - ENSG00000167553 [96%]
TUBA3C - ENSG00000198033 [100%]
   TUBA3D - ENSG00000075886 [100%]
TUBA1B - ENSG00000123416 [100%]
TUBA4A - ENSG00000127824 [100%]
TUBA3E - ENSG00000152086 [93%]
TUBA1A - ENSG00000167552 [100%]
TUBA1C - ENSG00000167553 [97%]
TUBAL3 - ENSG00000178462 [93%]
TUBA3C - ENSG00000198033 [100%]
    

Released in version

 10   13   9  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA043684

  

Antibody HPA063394

  

Antibody CAB046031

  
Immunocytochemistry

Supported      
Immunohistochemistry

Approved   Approved   Approved  
Western blot

Supported   Supported   Supported  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA043684

  

Antibody HPA063394

  

Antibody CAB046031

  

Standard validation

 Supported      

     

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to microtubules.      

Antibody dilution

 1:75      

Literature conformity

 The subcellular location is supported by literature.      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA043684

  

Antibody HPA063394

  

Antibody CAB046031

  

Standard validation

 Approved   Approved   Approved  

     

Figure description

 Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in cells of the seminiferous ducts.   Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.   Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.  

Expression

 RNA: detected in 35 tissues
Protein: detected in 41 cell types		   RNA: detected in 35 tissues
Protein: detected in 71 cell types		   RNA: detected in 35 tissues
Protein: detected in 75 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:150   1:40   1:18000  

Literature conformity

 Consistent with gene/protein characterization data.   Consistent with gene/protein characterization data.   Consistent with gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA043684

  

Antibody HPA063394

  

Antibody CAB046031

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 57.7, 52, 50.2, 50.1, 50, 49.9, 48.3, 46.3, 46.1, 43, 31, 17.7   57.7, 52, 50.2, 50.1, 50, 49.9, 48.3, 46.3, 45.5, 43, 31   52, 50.1, 43, 31, 5.3  

Antibody dilution

 1:250   1:80   1:500  

PROTEIN ARRAY
 
 

Antibody HPA043684

  

Antibody HPA063394

  

Antibody CAB046031

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:500    

ANTIGEN INFORMATION
 
 

Antibody HPA043684

  

Antibody HPA063394

  

Antibody CAB046031

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 24   29    

Antigen sequence

 
ATYAPVISAEKAYHEQLSVAEITN
  
NTTAIAEAWARLDHKFDLMYAKRAFVHWY
  

											 
																				

																		

										
											
Matching transcripts

												
																					TUBA8-001 - ENSP00000333326 [88%] 
TUBA8-002 - ENSP00000318575 [88%] 
TUBA8-003 - ENSP00000412646 [88%] 
TUBA8-005 - ENSP00000477306 [88%] 

											 
																						TUBA8-001 - ENSP00000333326 [100%] 
TUBA8-002 - ENSP00000318575 [100%] 
TUBA8-003 - ENSP00000412646 [100%] 
TUBA8-005 - ENSP00000477306 [93%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					TUBA3D - ENSG00000075886 [100%]
TUBA1B - ENSG00000123416 [100%]
TUBA4A - ENSG00000127824 [100%]
TUBA3E - ENSG00000152086 [100%]
TUBA1A - ENSG00000167552 [100%]
TUBA1C - ENSG00000167553 [96%]
TUBA3C - ENSG00000198033 [100%]

											 
																						TUBA3D - ENSG00000075886 [100%]
TUBA1B - ENSG00000123416 [100%]
TUBA4A - ENSG00000127824 [100%]
TUBA3E - ENSG00000152086 [93%]
TUBA1A - ENSG00000167552 [100%]
TUBA1C - ENSG00000167553 [97%]
TUBAL3 - ENSG00000178462 [93%]
TUBA3C - ENSG00000198033 [100%]

											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
TUBA8-001
TUBA8-002
TUBA8-003
TUBA8-005
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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