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TUBA3C

ANTIBODY INFORMATION
 
 

Antibody HPA039247

  

Antibody HPA043684

  

Antibody HPA063394

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA039247   HPA043684   HPA063394  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

 TUBA3D - ENSG00000075886 [100%]
TUBA1B - ENSG00000123416 [100%]
TUBA4A - ENSG00000127824 [96%]
TUBA3E - ENSG00000152086 [100%]
TUBA1A - ENSG00000167552 [100%]
TUBA1C - ENSG00000167553 [100%]
   TUBA3D - ENSG00000075886 [100%]
TUBA1B - ENSG00000123416 [100%]
TUBA4A - ENSG00000127824 [100%]
TUBA3E - ENSG00000152086 [100%]
TUBA1A - ENSG00000167552 [100%]
TUBA1C - ENSG00000167553 [96%]
TUBA8 - ENSG00000183785 [88%]
   TUBA3D - ENSG00000075886 [100%]
TUBA1B - ENSG00000123416 [100%]
TUBA4A - ENSG00000127824 [100%]
TUBA3E - ENSG00000152086 [93%]
TUBA1A - ENSG00000167552 [100%]
TUBA1C - ENSG00000167553 [97%]
TUBAL3 - ENSG00000178462 [93%]
TUBA8 - ENSG00000183785 [100%]
  

Released in version

 10   10   13  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA039247

  

Antibody HPA043684

  

Antibody HPA063394

  
Immunocytochemistry

Supported   Supported    
Immunohistochemistry

Uncertain   Uncertain   Uncertain  
Western blot

Supported   Supported   Supported  
Protein array

Supported   Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA039247

  

Antibody HPA043684

  

Antibody HPA063394

  

Standard validation

 Supported   Supported    

     

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to microtubules.   Immunofluorescent staining of human cell line U-2 OS shows localization to microtubules.    

Antibody dilution

 1:7   1:75    

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA039247

  

Antibody HPA043684

  

Antibody HPA063394

  

Standard validation

 Uncertain   Uncertain   Uncertain  

     

Figure description

 Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells of the seminiferous ducts.   Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in cells of the seminiferous ducts.   Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.  

Expression

 RNA: detected in 1 tissues
Protein: detected in 79 cell types		   RNA: detected in 1 tissues
Protein: detected in 41 cell types		   RNA: detected in 1 tissues
Protein: detected in 71 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:600   1:150   1:40  

Literature conformity

 Partly consistent with gene/protein characterization data.   Consistent with gene/protein characterization data.   Consistent with gene/protein characterization data.  

RNA consistency

 Not consistent with RNA expression data.   Not consistent with RNA expression data.   Not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA039247

  

Antibody HPA043684

  

Antibody HPA063394

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 57.7, 50.2, 50.1, 50, 49.9, 48.3, 46.1, 27.6, 24.8, 21.7, 19.8, 16.9, 14.4, 13.2   57.7, 52, 50.2, 50.1, 50, 49.9, 48.3, 46.3, 46.1, 43, 31, 17.7   57.7, 52, 50.2, 50.1, 50, 49.9, 48.3, 46.3, 45.5, 43, 31  

Antibody dilution

 1:250   1:250   1:80  

PROTEIN ARRAY
 
 

Antibody HPA039247

  

Antibody HPA043684

  

Antibody HPA063394

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500   1:3000   1:500  

ANTIGEN INFORMATION
 
 

Antibody HPA039247

  

Antibody HPA043684

  

Antibody HPA063394

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 28   24   29  

Antigen sequence

 
LEHGIQPDGQMPSDKTIGGGDDSFNTFF
  
ATYAPVISAEKAYHEQLSVAEITN
  
NTTAIAEAWARLDHKFDLMYAKRAFVHWY
 

Matching transcripts

 TUBA3C-002 - ENSP00000382982 [100%]
TUBA3C-201 - ENSP00000482878 [100%]
  TUBA3C-002 - ENSP00000382982 [100%]
TUBA3C-201 - ENSP00000482878 [100%]
  TUBA3C-002 - ENSP00000382982 [100%]
 

Other gene match

 TUBA3D - ENSG00000075886 [100%]
TUBA1B - ENSG00000123416 [100%]
TUBA4A - ENSG00000127824 [96%]
TUBA3E - ENSG00000152086 [100%]
TUBA1A - ENSG00000167552 [100%]
TUBA1C - ENSG00000167553 [100%]
   TUBA3D - ENSG00000075886 [100%]
TUBA1B - ENSG00000123416 [100%]
TUBA4A - ENSG00000127824 [100%]
TUBA3E - ENSG00000152086 [100%]
TUBA1A - ENSG00000167552 [100%]
TUBA1C - ENSG00000167553 [96%]
TUBA8 - ENSG00000183785 [88%]
   TUBA3D - ENSG00000075886 [100%]
TUBA1B - ENSG00000123416 [100%]
TUBA4A - ENSG00000127824 [100%]
TUBA3E - ENSG00000152086 [93%]
TUBA1A - ENSG00000167552 [100%]
TUBA1C - ENSG00000167553 [97%]
TUBAL3 - ENSG00000178462 [93%]
TUBA8 - ENSG00000183785 [100%]
  
ANTIGEN VIEW
TUBA3C-002
TUBA3C-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.