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Term
Gene name

Class

Subclass

Chromosome

External id

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Reliability

Reliability

Reliability

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Method

Validation

Method

Validation

Method

Validation

Location

Searches

Tissue

Cell type

Expression

Tissue

Specificity

Cell line

Specificity

Tissue

Detectable

Cell line

Detectable

Category

Score

Score

Score

Antibodies

Column

 
 
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ANTIBODY INFORMATION VALIDATION SUMMARY IMMUNOCYTOCHEMISTRY IMMUNOHISTOCHEMISTRY WESTERN BLOT PROTEIN ARRAY ANTIGEN INFORMATION RELEVANT PUBLICATIONS

ANTIBODY INFORMATION

 
 

Antibody HPA049840

 

Antibody HPA062236

 

Provider

Atlas Antibodies
Sigma-Aldrich
  Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA049840   HPA062236  

Host species

Rabbit   Rabbit  

Clonality

pAb   pAb  

Purity

Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

   

Released in version

10   13  

References

   

VALIDATION SUMMARY

 
 

Antibody HPA049840

 

Antibody HPA062236

 
Immunocytochemistry

 

Validated
 
Immunohistochemistry


Validated
   
Western blot

Supported  

Validated
 
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY

 
 

Antibody HPA049840

 

Antibody HPA062236

 

Formal validation: Genetic

 
Validated

Significant downregulation > 25% by both siRNA:s.
 

 
 

Relative fluorescence intensity (RFI)

 

Figure description

  Nuclear region of segmented cells in 10x-images  

Antibody dilution

  1:200  
 

Standard validation

  Supported  

   

Figure description

  Immunofluorescent staining of human cell line RH-30 shows localization to nuclear membrane.  

Antibody dilution

  1:200  

Literature conformity

  The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY

 
 

Antibody HPA049840

 

Antibody HPA062236

 

Formal validation: Orthogonal

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.
   

 

 

   

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.    
 

Formal validation: Orthogonal

Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.
   

 

 

   

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.    
 

Standard validation

Supported    

   

Figure description

Immunohistochemical staining of human lymph node shows distinct nuclear membranous positivity in lymphoid cells.    

Expression

RNA: detected in 37 tissues
Protein: detected in 20 cell types
   

Retrieval

HIER pH6    

Antibody dilution

1:1500    

Literature conformity

Consistent with gene/protein characterization data.    

RNA consistency

Mainly consistent with RNA expression data.    

WESTERN BLOT

 
 

Antibody HPA049840

 

Antibody HPA062236

 

Formal validation: Genetic

 
Validated

Downregulation visible in both siRNA lanes
 

   

Figure description

  Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled
 

Target mass (kDa)

  70.7, 24.3  

Loading control

  Total protein image  

Antibody dilution

  1:700  
 

Standard validation

Supported

Band of predicted size in kDa (+/-20%) with additional bands present.
  Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).
 

   

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY405131)
  Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
 

Target mass (kDa)

70.7, 24.3   70.7, 24.3  

Antibody dilution

1:250   1:1050  

PROTEIN ARRAY

 
 

Antibody HPA049840

 

Antibody HPA062236

 

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.
  Supported

Pass with single peak corresponding to interaction only with its own antigen.
 

   

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

1:5000   1:12600  

ANTIGEN INFORMATION

 
 

Antibody HPA049840

 

Antibody HPA062236

 

Antigen

Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

71   90  

Antigen sequence

MPSRKFADGEVVRGRWPGSSLYYEVEILSHDSTSQLYTVKYKDGTELELK
ENDIKPLTSFRQRKGGSTSSS
 
EARREVEVKLTPLILKPFGNSISRYNGEPEHIERNDAPHKNTQEKFNLSQ
ESSYIATQYSLRPRREEVKLKEIDSKEEKYVAKELAVRTF
 

Matching transcripts

LBR-001 - ENSP00000272163 [100%]
LBR-002 - ENSP00000388059 [100%]
LBR-201 - ENSP00000339883 [100%]
  LBR-001 - ENSP00000272163 [99%]
LBR-002 - ENSP00000388059 [99%]
LBR-201 - ENSP00000339883 [99%]
 

Other gene match

   
ANTIGEN VIEW
LBR-001
LBR-002
LBR-201
 

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The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.