Cell atlas - LBR - The Human Protein Atlas

GENERAL INFORMATION
Gene name	LBR
Gene description	Lamin B receptor
Protein class	Disease related genes Plasma proteins Predicted intracellular proteins Predicted membrane proteins
Predicted localization	Intracellular,Membrane
Number of transcripts	4
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HUMAN PROTEIN ATLAS INFORMATION
Summary	Localized to the nuclear membrane.
RNA cell category	Expressed in all
Protein evidence	Evidence at protein level
Main location	Localized to the Nuclear membrane (validated)
siRNA	The location(s) are Validated based on siRNA mediated gene silencing.

DATA RELIABILITY
Reliability score	Validated based on 1 antibody. HPA062236
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ASSAYS

RNA expression

Human cells

siRNA

RNA EXPRESSION OVERVIEW
RNA cell line category: Expressed in all Organ Origin Category Expression Alphabetical Cell lines sorted after organ of phenotypic resemblance. Cell lines sorted after biological source for establishment. Cell lines sorted after the cell line category according to Cellosaurus. Cell lines sorted on descending RNA expression. Cell lines sorted alphabetically.

HUMAN CELLS

Summary

Localized to the nuclear membrane.

Main location

Nuclear membrane (validated)


	Toggle channels	Low High G1 S G2 M N/A

Antibody	Cell line	Location
HPA062236	HEK 293	Nuclear membrane
HPA062236	RH-30	Nuclear membrane
HPA062236	U-2 OS	Nuclear membrane

siRNA VALIDATION

Relative fluorescence intensity (RFI)

Antibody

HPA062236

siRNA validation

Validated: Significant downregulation > 25% by both siRNA:s.

Analysis performed in

Nuclear region of segmented cells in 10x-images

siRNA 1

siRNA 2

RFI

29%

25%

p-value

< 0.01


	Toggle channels	Low High G1 S G2 M N/A

Antibody	Sample	Cell line
HPA062236	siRNA 1	U-2 OS
HPA062236	siRNA 2	U-2 OS
HPA062236	scrambled	U-2 OS

GENE INFORMATION
Gene name	LBR (HGNC Symbol)
Synonyms	DHCR14B, TDRD18
Description	Lamin B receptor (HGNC Symbol)
Entrez gene summary	The protein encoded by this gene belongs to the ERG4/ERG24 family. It localized in the nuclear envelope inner membrane and anchors the lamina and the heterochromatin to the membrane. It may mediate interaction between chromatin and lamin B. Mutations of this gene has been associated with autosomal recessive HEM/Greenberg skeletal dysplasia. Alternative splicing occurs at this locus and two transcript variants encoding the same protein have been identified. [provided by RefSeq, Jul 2008]
Chromosome	1
Cytoband	q42.12
Chromosome location (bp)	225401502 - 225428925
Protein evidence	Evidence at protein level (all genes)
Ensembl	ENSG00000143815 (version 83.38)
Entrez gene	3930
UniProt	Q14739 (UniProt - Evidence at protein level)
neXtProt	NX_Q14739
Antibodypedia	LBR antibodies

PROTEIN BROWSER

LBR-001	LBR-002	LBR-003	LBR-201

PROTEIN INFORMATION
Splice variant	UniProt	Protein class	Gene ontology	Length & mass	Signal peptide (predicted)	Transmembrane regions (predicted)
LBR-001 ENSP00000272163 ENST00000272163	Q14739 [Direct mapping] Lamin-B receptor Show all	Predicted membrane proteins Prediction method-based Membrane proteins predicted by MDM MEMSAT3 predicted membrane proteins MEMSAT-SVM predicted membrane proteins Phobius predicted membrane proteins SCAMPI predicted membrane proteins SPOCTOPUS predicted membrane proteins THUMBUP predicted membrane proteins TMHMM predicted membrane proteins # TM segments-based 9TM proteins predicted by MDM Plasma proteins Disease related genes Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0003677 [DNA binding] GO:0005515 [protein binding] GO:0005521 [lamin binding] GO:0005635 [nuclear envelope] GO:0005639 [integral component of nuclear inner membrane] GO:0006695 [cholesterol biosynthetic process] GO:0016020 [membrane] GO:0016021 [integral component of membrane] GO:0016627 [oxidoreductase activity, acting on the CH-CH group of donors] GO:0016628 [oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor] GO:0031965 [nuclear membrane] GO:0044281 [small molecule metabolic process] GO:0044822 [poly(A) RNA binding] GO:0055114 [oxidation-reduction process] GO:0070087 [chromo shadow domain binding] Show all	615 aa 70.7 kDa	No	9
LBR-002 ENSP00000388059 ENST00000425080	C9JXK0 [Direct mapping] Lamin-B receptor Show all	Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all	GO:0031965 [nuclear membrane] Show all	213 aa 24.3 kDa	No	0
LBR-003 ENSP00000416554 ENST00000421383	C9JES9 [Direct mapping] Lamin-B receptor Show all	Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all		51 aa 5.9 kDa	No	0
LBR-201 ENSP00000339883 ENST00000338179	Q14739 [Direct mapping] Lamin-B receptor Show all	Predicted membrane proteins Prediction method-based Membrane proteins predicted by MDM MEMSAT3 predicted membrane proteins MEMSAT-SVM predicted membrane proteins Phobius predicted membrane proteins SCAMPI predicted membrane proteins SPOCTOPUS predicted membrane proteins THUMBUP predicted membrane proteins TMHMM predicted membrane proteins # TM segments-based 9TM proteins predicted by MDM Plasma proteins Disease related genes Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0003677 [DNA binding] GO:0005515 [protein binding] GO:0005521 [lamin binding] GO:0005635 [nuclear envelope] GO:0005639 [integral component of nuclear inner membrane] GO:0006695 [cholesterol biosynthetic process] GO:0016020 [membrane] GO:0016021 [integral component of membrane] GO:0016627 [oxidoreductase activity, acting on the CH-CH group of donors] GO:0016628 [oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor] GO:0031965 [nuclear membrane] GO:0044281 [small molecule metabolic process] GO:0044822 [poly(A) RNA binding] GO:0055114 [oxidation-reduction process] GO:0070087 [chromo shadow domain binding] Show all	615 aa 70.7 kDa	No	9