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Immunohistochemical staining of human cerebellum shows strong cytoplasmic and nuclear positivity in Purkinje cells and strong nuclear positivity in cells in molecular layer.
Immunohistochemical staining of human cerebellum shows strong nuclear and cytoplasmic positivity in Purkinje cells and strong nuclear in cells in molecular layer.
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in leydig cells.
Expression
RNA: detected in 16 tissues Protein: detected in 50 cell types
RNA: detected in 16 tissues Protein: detected in 57 cell types
RNA: detected in 16 tissues Protein: detected in 5 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH9
Antibody dilution
1:100
1:75
1:300
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Standard validation
Supported
Figure description
Cytoplasmic positivity in subsets of neurons as well as processes.
WESTERN BLOT
Antibody HPA007305
Antibody HPA007306
Antibody CAB000106
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY400659)
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
31.5
31.5, 27.2, 22.2
31.5, 27.2, 22.2, 18.4
Antibody dilution
1:250
1:250
1:500
PROTEIN ARRAY
Antibody HPA007305
Antibody HPA007306
Antibody CAB000106
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
