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Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in glandular cells with distinct extracellular material.
Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.
Expression
RNA: detected in 3 tissues Protein: detected in 37 cell types
RNA: detected in 3 tissues Protein: detected in 44 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:100
1:150
Literature conformity
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA036136
Antibody HPA036137
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY403899)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY403899)
Target mass (kDa)
21.6, 21.5
21.6, 21.5
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA036136
Antibody HPA036137
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
