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ACTA2

ANTIBODY INFORMATION
 
 

Antibody HPA041264

  

Antibody HPA041271

  

Antibody CAB000002

  

Antibody CAB013531

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   DakoCytomation
   Zymed
  

Product name

 HPA041264   HPA041271   M0851   18-0106  

Host species

 Rabbit   Rabbit   Mouse   Mouse  

Clonality

 pAb   pAb   mAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Supernatant   Not known  

Other gene match

 ACTB - ENSG00000075624 [100%]
ACTA1 - ENSG00000143632 [96%]
ACTC1 - ENSG00000159251 [96%]
ACTG2 - ENSG00000163017 [96%]
ACTBL2 - ENSG00000169067 [92%]
ACTG1 - ENSG00000184009 [100%]
POTEE - ENSG00000188219 [100%]
POTEF - ENSG00000196604 [100%]
POTEI - ENSG00000196834 [100%]
POTEJ - ENSG00000222038 [100%]
   ACTB - ENSG00000075624 [100%]
ACTA1 - ENSG00000143632 [97%]
ACTC1 - ENSG00000159251 [97%]
ACTG2 - ENSG00000163017 [97%]
ACTBL2 - ENSG00000169067 [92%]
ACTG1 - ENSG00000184009 [100%]
POTEE - ENSG00000188219 [84%]
POTEF - ENSG00000196604 [84%]
POTEI - ENSG00000196834 [84%]
POTEJ - ENSG00000222038 [84%]
      

Released in version

 10   10   1   4  

References

        

VALIDATION SUMMARY
 
 

Antibody HPA041264

  

Antibody HPA041271

  

Antibody CAB000002

  

Antibody CAB013531

  
Immunohistochemistry

Supported   Supported   Supported  

Validated
  
Western blot

Supported   Uncertain   Supported   Supported  
Protein array

Approved   Supported      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA041264

  

Antibody HPA041271

  

Antibody CAB000002

  

Antibody CAB013531

  

Formal validation: Orthogonal

      
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

     
 

 

  

Figure description

       Distribution of protein expression (antibody staining) and RNA expression (TPM) across 43 cell lines.  
 

Standard validation

 Supported   Supported   Supported   Supported  

       

Figure description

 Immunohistochemical staining of human stomach, upper shows strong membranous positivity in glandular cells.   Immunohistochemical staining of human stomach, upper shows strong membranous positivity in glandular cells.   Immunohistochemical staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.   Immunohistochemical staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 73 cell types		   RNA: detected in 37 tissues
Protein: detected in 37 cell types		   RNA: detected in 37 tissues
Protein: detected in 4 cell types		   RNA: detected in 37 tissues
Protein: detected in 8 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH9   HIER pH6  

Antibody dilution

 1:1800   1:150   1:200   1:100  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA041264

  

Antibody HPA041271

  

Antibody CAB000002

  

Antibody CAB013531

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

       

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		     Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 213, 118, 83, 50, 33, 26, 18
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 121.4, 121.3, 117.4, 42.1, 42, 41.9, 41.8, 41.7, 37.1, 28.2, 26.9, 23.8, 18, 16.8   121.4, 121.3, 117.4, 42.1, 42, 41.9, 41.8, 41.7, 28.2, 26.9, 23.8, 18, 16.8, 16.3, 14.5, 13, 11.4, 11, 8.5   42, 16.8   42, 16.8  

Antibody dilution

 1:250   1:250   1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA041264

  

Antibody HPA041271

  

Antibody CAB000002

  

Antibody CAB013531

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		      

       

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.      

Antibody dilution

 1:3000   1:3000      

ANTIGEN INFORMATION
 
 

Antibody HPA041264

  

Antibody HPA041271

  

Antibody CAB000002

  

Antibody CAB013531

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Synthetic peptide   Not known  

Length (aa)

 26   37      

Antigen sequence

 
PKANREKMTQIMFETFNTPAMYVAIQ
  
MVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWD
  

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					ACTA2-001 - ENSP00000224784 [96%] 
ACTA2-002 - ENSP00000396730 [96%] 
ACTA2-003 - ENSP00000398239 [96%] 
ACTA2-201 - ENSP00000402373 [96%] 

											 
																						ACTA2-001 - ENSP00000224784 [97%] 
ACTA2-002 - ENSP00000396730 [97%] 
ACTA2-003 - ENSP00000398239 [97%] 
ACTA2-201 - ENSP00000402373 [97%] 

											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					ACTB - ENSG00000075624 [100%]
ACTA1 - ENSG00000143632 [96%]
ACTC1 - ENSG00000159251 [96%]
ACTG2 - ENSG00000163017 [96%]
ACTBL2 - ENSG00000169067 [92%]
ACTG1 - ENSG00000184009 [100%]
POTEE - ENSG00000188219 [100%]
POTEF - ENSG00000196604 [100%]
POTEI - ENSG00000196834 [100%]
POTEJ - ENSG00000222038 [100%]

											 
																						ACTB - ENSG00000075624 [100%]
ACTA1 - ENSG00000143632 [97%]
ACTC1 - ENSG00000159251 [97%]
ACTG2 - ENSG00000163017 [97%]
ACTBL2 - ENSG00000169067 [92%]
ACTG1 - ENSG00000184009 [100%]
POTEE - ENSG00000188219 [84%]
POTEF - ENSG00000196604 [84%]
POTEI - ENSG00000196834 [84%]
POTEJ - ENSG00000222038 [84%]

											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
ACTA2-001
ACTA2-002
ACTA2-003
ACTA2-201
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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