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ACTB

ANTIBODY INFORMATION
 
 

Antibody HPA041264

  

Antibody HPA041271

  

Antibody CAB002621

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Lab Vision/NeoMarkers
  

Product name

 HPA041264   HPA041271   RB-9421  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

 ACTA2 - ENSG00000107796 [96%]
ACTA1 - ENSG00000143632 [96%]
ACTC1 - ENSG00000159251 [96%]
ACTG2 - ENSG00000163017 [96%]
ACTBL2 - ENSG00000169067 [92%]
ACTG1 - ENSG00000184009 [100%]
POTEE - ENSG00000188219 [100%]
POTEF - ENSG00000196604 [100%]
POTEI - ENSG00000196834 [100%]
POTEJ - ENSG00000222038 [100%]
   ACTA2 - ENSG00000107796 [97%]
ACTA1 - ENSG00000143632 [97%]
ACTC1 - ENSG00000159251 [97%]
ACTG2 - ENSG00000163017 [97%]
ACTBL2 - ENSG00000169067 [92%]
ACTG1 - ENSG00000184009 [100%]
POTEE - ENSG00000188219 [84%]
POTEF - ENSG00000196604 [84%]
POTEI - ENSG00000196834 [84%]
POTEJ - ENSG00000222038 [84%]
    

Released in version

 10   10   1  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA041264

  

Antibody HPA041271

  

Antibody CAB002621

  
Immunohistochemistry

Supported   Supported   Supported  
Western blot

Supported   Uncertain   Supported  
Protein array

Approved   Supported    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA041264

  

Antibody HPA041271

  

Antibody CAB002621

  

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human stomach, upper shows strong membranous positivity in glandular cells.   Immunohistochemical staining of human stomach, upper shows strong membranous positivity in glandular cells.   Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in reaction center cells  

Expression

 RNA: detected in 37 tissues
Protein: detected in 73 cell types		   RNA: detected in 37 tissues
Protein: detected in 37 cell types		   RNA: detected in 37 tissues
Protein: detected in 64 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:1800   1:150   1:400  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA041264

  

Antibody HPA041271

  

Antibody CAB002621

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		     Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 121.4, 121.3, 117.4, 42.1, 42, 41.9, 41.8, 41.7, 37.1, 28.2, 26.9, 23.8, 18, 16.8   121.4, 121.3, 117.4, 42.1, 42, 41.9, 41.8, 41.7, 28.2, 26.9, 23.8, 18, 16.8, 16.3, 14.5, 13, 11.4, 11, 8.5   41.7, 18, 11.4, 11, 8.5  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA041264

  

Antibody HPA041271

  

Antibody CAB002621

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA041264

  

Antibody HPA041271

  

Antibody CAB002621

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Not known  

Length (aa)

 26   37    

Antigen sequence

 
PKANREKMTQIMFETFNTPAMYVAIQ
  
MVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWD
   

Matching transcripts

 ACTB-001 - ENSP00000349960 [100%]
ACTB-008 - ENSP00000407473 [100%]
  ACTB-001 - ENSP00000349960 [100%]
ACTB-008 - ENSP00000407473 [100%]
ACTB-009 - ENSP00000393951 [100%]
ACTB-010 - ENSP00000401032 [100%]
ACTB-012 - ENSP00000399487 [100%]
   

Other gene match

 ACTA2 - ENSG00000107796 [96%]
ACTA1 - ENSG00000143632 [96%]
ACTC1 - ENSG00000159251 [96%]
ACTG2 - ENSG00000163017 [96%]
ACTBL2 - ENSG00000169067 [92%]
ACTG1 - ENSG00000184009 [100%]
POTEE - ENSG00000188219 [100%]
POTEF - ENSG00000196604 [100%]
POTEI - ENSG00000196834 [100%]
POTEJ - ENSG00000222038 [100%]
   ACTA2 - ENSG00000107796 [97%]
ACTA1 - ENSG00000143632 [97%]
ACTC1 - ENSG00000159251 [97%]
ACTG2 - ENSG00000163017 [97%]
ACTBL2 - ENSG00000169067 [92%]
ACTG1 - ENSG00000184009 [100%]
POTEE - ENSG00000188219 [84%]
POTEF - ENSG00000196604 [84%]
POTEI - ENSG00000196834 [84%]
POTEJ - ENSG00000222038 [84%]
    
ANTIGEN VIEW
ACTB-001
ACTB-008
ACTB-009
ACTB-010
ACTB-012
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.