Validation of antibodies using GFP-tagged target

Cell Atlas Co-localization HPA14 Immunocytochemistry Immunofluorescence Subcell Atlas Validation

Antibody HPA37362 targeting BOD1L1 overlaps with GFP-tagged target protein and the localization is identical to that of endogenous protein in non-transfected control cells

Version 14 of The Human Protein Atlas includes a new type of validation of antibodies that are used for determining the subcellular localization of a protein.

A set of antibodies have been analyzed in transgenic cell lines expressing GFP-tagged target protein at near-endogenous levels to confirm that the antibodies are capable of binding the target protein. The approved antibodies are then used to determine the subcellular localization of endogenous protein in a selection of cell lines. A high validation score is assigned to those genes where the same location(s) are observed for both tagged protein and protein detected using labelled antibody in non-transfected cells...Read more

New investment in Life Science research

AstraZeneca Life Science Wallenberg WCPR

Great news for Life Science researchers in Sweden and the new Wallenberg Center for Protein Research (WCPR).

AstraZeneca and the Knut and Alice Wallenberg Foundation support a new center for protein research, the Wallenberg Center for Protein Research (WCPR). Three of Swedens top Universities, KTH - Royal Institute of Technology, Uppsala University, and Chalmers Technical Univeristy, will work together to improve human health by developing new biological molecules, antibodies and peptides...Read more

Solid-phase cloning for high-throughput assembly

DNA Expression vector High-throughput cloning

Attachment of DNA fragments onto a growing strand of DNA linked to a paramagnetic bead

Solid-phase cloning for high-throughput assembly of single and multiple DNA parts

Simple and automated workflows for DNA assembly are necessary in large-scale projects such as the Human Protein Atlas. For this reason, we have developed two automated methods for swift and convenient high-throughput assembly of DNA into expression vector constructs.

By using paramagnetic beads as a support for the DNA, we can perform construct assembly on a liquid handler robot and furthermore eliminate the need for prior purification of PCR products by spin or gel purification...Read more

A prognosis based classification of uterin cancer

Cancer Microarray Tissue Atlas Undifferentiated uterine sarcoma

The ovaries, fallopian tubes, and uterus

A prognosis based classification of undifferentiated uterine sarcomas: Identification of mitotic index, hormone receptors and YWHAE-FAM22 translocation status as predictors of survival

Undifferentiated uterine sarcomas (UUS) are rare tumors with a heterologous biology and a poor prognosis.

In a recent study by scientists from the Human Protein Atlas project and colleagues, the relevance of clinicopathology, mitotic index, translocation status (YWHAE-FAM22), and a number of biomarker candidates were examined for correlation with the prognosis of these tumors. The protein biomarkers P53, P16, Ki-67, Cyclin-D1, ER, PR, and ANLN were evaluated by immunohistochemistry...Read more

Human Protein Atlas talks and posters in December

Poster Presentation

  • December 1-2

    Human Quantitative Dynamics

    Washington DC, USA

    Presenter: Mathias UhlÚn

    Talk: The Human Protein Atlas

    Human Quantitative Dynamics

  • December 3-6

    The 7th Annual Meeting of Proteomic Society, India - 2015

    Vellore, India

    Presenter: Prof. Peter Nilsson, KTH Royal Institute of Technology, Sweden

    Talk: Human Protein Atlas enabled neuroproteomic profiling of body fluids

    The 7th Annual Meeting of Proteomic Society

  • December 10, 4:40 PM

    Salamanca, Spain

    Dr. Jochen M...Read more

    The human cardiac and skeletal muscle proteomes

    Heart Proteome Tissue Atlas

    The human cardiac and skeletal muscle proteomes defined by transcriptomics and antibody-based profiling

    To understand cardiac and skeletal muscle function, it is important to define and explore their molecular constituents to identify similarities and differences in gene expression patterns between these different striated muscle tissues. In an effort driven by scientists from the Human Protein Atlas and colleagues, the genes and proteins with elevated expression in cardiac and skeletal muscle in relation to all other major human tissues and organs were investigate using a global transcriptomics analysis complemented with antibody-based profiling...Read more

    Prognostic impact of COX-2 in lung cancer

    Cancer COX-2 Lung cancer Microarray Tissue Atlas

    COX-2 expression in stroma

    In a study performed by scientists at Uppsala University, in collaboration with Gothenburg University and the Human Protein Atlas project, the expression of cyclooxygenase-2 (COX-2) was evaluated in patients with non-small cell lung cancer (NSCLC).

    To clarify previous inconsistences concerning the prognostic impact of COX-2 expression in NSCLC, the association between COX-2 transcript levels (encoded by the gene PTGS2) and overall survival in nine publicly available gene expression microarray data sets were evaluated as well as the in situ protein expression of COX-2 in tumor and stromal cells in two independent NSCLC cohorts...Read more

    Increasing reliability by co-localization

    Cell Atlas Co-localization HPA14 Immunocytochemistry Immunofluorescence Subcell Atlas Validation

    Subcellular localization obtained using IF (left) and GFP (FP, right) for the endoplasmic protein FKBP7 (top) and the FHL2 protein localized to focal adhesion sites (bottom).

    Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells

    The Human Protein Atlas applies antibodies for a variety of applications to map protein expression in different tissues and also at the subcellular level. Within the subcellular protein atlas, immunofluorescence (IF) is used to uncover the localization of proteins to different organelles. To ensure an accurate localization of each and very protein, the antibodies have to be specific to their target protein...Read more

    Elevated expression of CD93 in brain tumor

    Brain cancer Cancer CD93 Glioblastoma Tissue Atlas

    CD93 expression in pathological blood vessels in a case of high-grade malignant glioma

    Elevated expression of the C-type lectin CD93 in the glioblastoma vasculature regulates cytoskeletal rearrangements that enhance vessel function and reduce host survival

    CD93 encodes for a cell-surface glycoprotein and is expressed in myeloid cells and endothelial cells. CD93 has recently been identified as an important gene in primary tumor angiogenesis and the corresponding protein has been shown to have proangiogenic properties. In the present study the role of CD93 in malignant glioma was analyzed. The effect on glioblastoma vessels and tumor growth was studied...Read more

    Human Protein Atlas presentations in November


  • November 2, 2:30 PM
  • 10th International MicroRNAs/Non-Coding RNAs & Genome Editing Europe 2015 Meeting

    4th International European Neurodegenerative Diseases/Optogenetics Europe 2015 Meeting

    Cambridge, United Kingdom

    Presenter: Prof. Peter Nilsson, KTH Royal Institute of Technology

    Talk: Human Protein Atlas enabled neuroproteomic profiling of body fluids


  • November 18-21
  • Blood and Skin - new perspectives for neglected diseases

    Addis Ababa, Ethiopia

    Presenter: Prof...Read more

    The human brain proteome

    Brain Proteome Tissue Atlas

    The mammalian brain is a complex organ composed of many specialized cells, as well as specialized and discretely localized proteins. In a recent study by scientists from the Human Protein Atlas and colleagues, transcriptomics and protein expression data was used to analyze brain-enriched genes from the frontal cortex. Based on transcriptomics analysis of altogether 27 tissues, it was estimated that approximately 3% of all protein coding genes and 13% of the long non-coding genes expressed in the human brain are enriched (having at least five times higher expression levels in brain as compared to any of the other analyzed tissues)...Read more

    The building blocks of life

    HPA13 Science Tissue Atlas

    All major human tissues and organs analyzed for protein expression.

    Tissue-based map of the human proteomeThe effort to map the human proteome gives new insights on human health and disease.

    The Human Protein Atlas, a large-scale multidisciplinary project supported by the Knut and Alice Wallenberg foundation in Sweden, is mapping all human proteins to their location in the body. The project, which was initiated a decade ago, has engaged researchers from many different disciplines and to date annotated over 13 million images showing the localization of our proteins in the human body on a cellular and sub-cellular level. All images and data are publicly available at the web portal Human Protein Atlas...Read more

    Release of HPA14 and the Human Protein Atlas blog

    HPA14 Mouse Brain Atlas Release

    Protein distribution in a single brain section of the mouse brain down to the single cell level.

    Focus in version 14 has been to improve validation of the antibodies used to map the human proteome and the inclusion of a new atlas; the Mouse Brain Atlas created by the Fluorescence Tissue Profiling facility at Science for Life Laboratory (SciLifeLab) in Stockholm.

    Many of the mouse proteins have extensive homology with the human counterpart and this forms the basis for using the mouse brain as a model for the corresponding human brain to explore the expression and distribution of proteins in the various regions and cells of the brain...Read more

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