THE HUMAN PROTEIN ATLAS BLOG

Proteomic analysis of cell cycle progression reveal mitotic substages

2017-11-02
Affinity proteomics Cell Atlas Cell cycle Immunofluorescence

Examples of spindle phenotypes (Bipole, Collapsed, Multipole and Monopole)

A key feature and a critical first step in understanding cell division and proliferation lies in characterizing the temporal regulation of protein abundance. A collaborative publication "Proteomic analysis of cell cycle progression in asynchronous cultures, including mitotic subphases, using PRIMMUS" was recently published in eLife.

The Cell atlas team from Sweden joined forces with Dr Tony Ly and Professor Angus Lamond from the University of Dundee, to perform a proteome-wide analysis of changes in protein abundance and phosphorylation across the cell cycle...Read more


Proteomics methods promote understanding autoimmunity in neurodegenerative disorders

2017-10-09
Affinity proteomics blood samples Malaria Multiple Sclerosis narcolepsy Neurodegenerative disorders Plasma Plasma Profilling psychosis

Arash Zandian's doctoral thesis defense at SciLifeLab, KTH

The last decades of technical development and availability of protein and peptide microarrays have enabled large-scale profiling of antibodies and precise determination of their specificities through epitope mapping. This has enabled characterization of the human immune response and the produced antibodies. Beside exploring the auto-antibody repertoire, this approach also identifies key autoantigens targeted by the antibodies in e.g. neurodegenerative disorders...Read more


Human Protein Atlas presentations at the 16th HUPO World Congress

2017-09-14
Affinity proteomics Cell Atlas Conference Human Protein Atlas Immunofluorescence microscopy Immunohistochemistry Sub-cellular structures Tissue Atlas

Next week on the 17th to 21th of September, the human proteome organization (HUPO), is hosting the 16th HUPO World Congress in Dublin, Ireland. Several Human Protein Atlas-associated researchers will attend the meeting and represent the project in various sessions, including plenary and invited lectures, oral presentations and poster sessions.

HUPO is an international scientific organization representing and promoting proteomics through international cooperation and collaborations by fostering the development of new technologies, techniques and training...Read more


Epitope mapping and development of therapeutic antibodies

2017-04-11
Affinity proteomics Antibody Antibody Microarray cells Epitope mapping Human Protein Atlas Life Science

Johan Rockberg

Within the Human Protein Atlas project, antibodies are used to study the localization of protein in human tissues and cells. To generate the antibodies recombinant expression clones are produced from human RNA pools by cDNA synthesis, cloning and plasmid purification. These clones produce what is called Protein Epitope Signature Tags (PrESTs), a selected part of the target protein that should be recognized by antibodies.

Johan Rockberg, Associate Professor in antibody technology and directed evolution at KTH - Royal Institute of Technology in Stockholm, Sweden is the group leader for the epitope mapping and therapeutic antibodies group within the Human Protein Atlas...Read more


They are best at translating research to market

2017-02-28
Affinity proteomics Antibody

This month, the online magazine Labiotech.eu writes about scientists who translates science from academia to the market. While there are a many academic entrepreneurial superstars in the US, Labiotech.eu points out that there are also some academic researchers in Europe that have been successful in taking their findings into the biotech industry and lists ten individuals in Europe who have been successful at co-founding biotech companies.

Out of these ten persons, no less than three have connections to Sweden. Emanuelle Charpentier, co-discoverer of CRISPR, did some of her findings at Umeň University...Read more


Wellness profiling for early detection of disease

2017-01-17
Affinity proteomics Biobank profiling Biomarkers

www.inasakvareller.se

During the Precision Medicine World Conference in Silicon Valley on January 23-25, Mathias Uhlen, leader of the Human Protein Atlas will give a talk about the recently initiated SCAPIS – SciLifeLab Wellness Profiling project.

The Precision Medicine World Conference is a conference series that attracts recognized leaders, top global researchers and medical professionals, and innovators across healthcare and biotechnology sectors to showcase practical content that helps close the knowledge gap between different sectors, thereby catalyzing cross-functional fertilization and collaboration...Read more


Affinity proteomics for plasma biomarker screening

2016-10-18
Affinity proteomics Biomarkers Plasma

Graphical representation of the study

In a recent study, published in Blood, researchers have used affinity reagents from the Human Protein Atlas project to analyze plasma samples to identify candidate protein markers associated with risk of venous thromboembolism.

Venous thromboembolism is the third most common cardiovascular disease and a leading cause of death and disability worldwide. Complex interactions between genetic, environmental and acquired risk factors underlie disease development. The first step in the process of developing clinically applicable predictive tools is the identification of novel markers that associate with the disease...Read more


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